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Technology Platforms

 
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A

Advanced Bioimaging Centre
www.sickkids.ca/…/Advanced-Bioimaging-Centre/

The Advanced Bioimaging Centre is a joint facility of Mount Sinai Hospital and The Hospital for Sick Children Research Institute. It is located in the Department of Pathology & Laboratory Medicine at Mount Sinai Hospital and provides microscopy services to scientists at both institutions as well as the University of Toronto and the scientific community at large.

The Centre combines state-of-the-art equipment and technical expertise in a user-friendly environment. 

Technical Contact: Doug Holmyard 
Scientific staff and Co-directors:
Dr. David P. Bazett-Jones
Dr. Marc D. Grynpas 
Associate: Dr. John Rubinstein


B

BioBank
biobank.lunenfeld.ca

BioBank of the Research Centre for Women’s and Infants’ Health (RCWIH) – The RCWIH BioBank is a biorepository established to support perinatal research programs by providing access to normal and pathological tissue specimens and associated clinical data. The RCWIH BioBank operates on a fee-for-service (cost recovery) basis. The program has ethics approval from the Mount Sinai Hospital Research Ethics Board, and is overseen by the RCWIH BioBank Governance Committee. RCWIH BioBank staff are dedicated to the procurement, processing and archival of fresh, frozen and fixed specimens for a wide range of applications in perinatal research. Collected specimens include placental tissue and membranes, umbilical cord tissue and cord blood, and parental samples (blood, saliva, buccal cells). The RCWIH BioBank is a program of the Lunenfeld-Tanenbaum Research Institute and the Mount Sinai Hospital/University Health Network Department of Obstetrics & Gynaecology.

PI: Dr S. Lee Adamson (RCWIH BioBank Director)

 

Biospecimen Repository
research.lunenfeld.ca/bsr

The Biospecimen Repository and Processing Lab provides investigators within and outside the LTRI, with accurate, well annotated, high quality biospecimen processing, storage and shipment for their research needs.

We provide services to process biospecimens such as blood, saliva, urine, tumour tissue (fresh frozen or paraffin embedded tissue) as well as isolate DNA or RNA from these source samples. The fully automated robotic aliquoting system is available for aliquoting biospecimens for large scale studies. Manual aliquoting and processing systems are used for smaller studies which allows for customization. Our robotic DNA extraction system can be utilized for large sample size and manual extraction protocols are available when smaller DNA yields, such as paraffin embedded tissue, is expected.

The laboratory and freezer storage facility occupies 2,808 square feet and has capacity to house 21 large liquid nitrogen freezers and 17 are in use. Liquid nitrogen is piped in from a bulk tank outside the building though vacuum jacketed piping. All freezers have internal alarms that display temperature and liquid nitrogen levels as well as an external freezer monitoring alarm system that alert our staff 24 hours a day 7 days per week thus allowing immediate response. By having controlled access to the freezers, sample integrity is maintained at all times. Back up freezers are available should one fail.

The laboratory complies with laboratory accreditation programs thus all our procedures are documented and adhered to following established programs. All documents are controlled through a document management system.  

Facility contact: Teresa Selander 

 


 

C

Flow Cytometry
flowcytometry.lunenfeld.ca

The Lunenfeld-Tanenbaum Research Institute has two flow cytometry facilities offering a full compliment of leading edge instrumentation from cell-sorting and analysis to the latest in imaging flow technology. We are a full service organization serving investigators at the Lunenfeld-Tanenbaum along with external clients both academic and corporate. Through our strategic partnership with Mount Sinai Services (MSS) a full range of commercial services are available from high-throughput analytical and diagnostic testing to clinical trial management. Key partnerships with Beckman Coulter, Amnis and Lunenfeld-Tanenbaum Robotics provide added specialization in developing advanced, customized applications with high throughput potential.

Locations:
Rm. L4-420, 25 Orde street
Rm. 980, Mount Sinai Hospital (Lunenfeld-Tanenbaum)

Facility Contact: Michael Parsons 

 


 

E

ES Cell Core

A centralized facility for the handling and manipulation of mouse ES cells. The Core provides the users from Lunenfeld-Tanenbaum laboratories with quality-controlled ES cells developed at Lunenfeld-Tanenbaum as well as reagents necessary for the experiments. Currently R1 and G4 ES cells as well as Mouse Embryonic fibroblast (MEF) prepared from TgN (DR4) 1 Jae strain that are resistant to G418, puromycin, hygromycin, and 6-thioguanine are available for use in the facility. The pool of ES cells and Mouse Embryonic Fibroblasts (MEF) used by the core has been MAP tested free of a range of mouse pathogens and Mycoplasma.

PI Contact: Dr. Andras Nagy
Facility contact: Malgosia Kownacka 

 


 

H

Core Histology

at 25 Orde St.

Histology lab equipment: automated slide stainer and coverslipper, automated tissue processor, embedding station, motorized/manual microtome and a motorized/manual cryostat.

Lunenfeld-Tanenbaum Contact: Dr. Mark Toone, Director Research Operations
Facility contact: Ryszard Bielecki – Bielecki@lunenfeld.ca


M

DNA Microarray Facility
research.lunenfeld.ca/microarray

Services and a variety of microarray products including mouse, human, yeast, C. elegans and zebra fish arrays.

PI contact: Dr. Chi Yip Ho
Facility contact: Kin Chan  

 

MITACS
research.lunenfeld.ca/mitacs


N

Neurobehaviour Core
neurobehaviour.lunenfeld.ca

A facility within the Lunenfeld-Tanenbaum that gives researchers access to a state-of-the-art phenotyping facility within the Institute, quick and easy access to all the costs involved, protocols, specific score sheets, and useful information and links for each test. The Neurobehaviour Core site allows staff to select tests to run and make arrangements to be trained on the protocol and equipment properly; set up meetings with core staff who can assist with any difficulties that may arise, and; book consultation meetings before/after testing mice, to help select the appropriate test or analyze the data.

Core contacts: Tatiana Lipina and Igor Vukobradovic
Facility contact: Annette Erlich

 

Network Biology Collaborative Centre
nbcc.lunenfeld.ca

The Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute is dedicated to helping scientists couple the vast knowledge of genomic and phenotypic variation in health and disease to a functional understanding of how gene products convey biological information and how their alterations drive disease pathologies. The Centre is composed of integrated state-of-the-art facilities enabling high-throughput, high-content screening and comprehensive mass spectrometry analysis.

PI Contact: Dr. Anne-Claude Gingras and Dr. Jeff Wrana
Facility contact: Karen Colwill


   
O

OpenFreezer at the Lunenfeld-Tanenbaum
openfreezer.lunenfeld.ca

OpenFreezer at the Lunenfeld-Tanenbaum is a reagent and location tracking software created in the Pawson lab that is available to all researchers at the institute. Users of OpenFreezer Lunenfeld-Tanenbaum can track information on all their reagents such as types of reagents, specific properties of each reagent (including sequences), different isolates and physical preparations (preps) of a reagent, and where these preps are stored in the laboratory. Importantly, OpenFreezer is the portal to the Institute’s clone repository that contains over 65,000 mouse and human cDNAs. To access the clone repository and enter reagents, users should contact Marina or Karen for a username and password.

Software Developer: Marina Olhovsky
Project Manager: Dr. Karen Colwill

 

Optical Imaging Facility (OPTIMA)
research.lunenfeld.ca/optima/

The OPTIMA Facility provides expert advice and maintains Microscopy Imaging and Analysis systems. The instruments are used primarily to image fluorochromes and histological stains, though brightfield and reflected light images can be collected. Equipment range from microscopes equipped with cameras for fluorescence and transmitted light imaging to laser-scanning/live imaging systems. There are several automated high-content screening systems for imaging multi-well plates at low- or high-resolution. We train users to perform their own acquisition and analysis, yet also provide staff-assisted imaging services.

High-Content Screening Facility

MSH site (Rm. 865)
PI contact: Dr. Laurence Pelletier
Facility contact: Dr. Mikhail Bashkurov
 

Microscopy Imaging

MSH site (Rm. 865)
PI contact: Dr. Joseph Culotti
Facility contacts: Louise Brown, John Georgiou, Sarang Kulkarni
imaging@lunenfeld.ca
 

Microscopy Imaging

Orde site
Facility contact: Ryszard Bielecki – Bielecki@lunenfeld.ca

 


 

P

Toronto Centre for Phenogenomics (TCP)
www.phenogenomics.ca

A state-of-the-art facility for the production, housing, and preclinical analysis of genetically altered mice. The TCP is a unique collaboration between Mount Sinai Hospital and The Hospital for Sick Children.

TCP Transgenic Core Facility (TG Core)
http://www.phenogenomics.ca/transgenics/

An amalgamation of the Transgenic Facilities at Mount Sinai Hospital and The Hospital for Sick Children that has pioneered innovative technologies to create genetically engineered model organisms. The TG Core serves resident TCP research programs, TCP member institutes, and laboratories around Toronto and internationally.

The Centre for Modeling Human Disease
www.phenogenomics.ca/services/phenotyping.html

Mouse models relevant to human disease & screening tools for in-depth analysis of the major physiological systems in the mouse.

Contact: Dr. Lise Phaneuf, Director, Research & Facility Operations
 

Proteomics and Mass Spectrometry
proteomics.lunenfeld.ca

Consists of a variety of mass spectrometers and separation systems and was expanded in 2010. State-of-the-art instrumentation in the Lunenfeld-Tanenbaum’s Proteomics Technology Centre greatly facilitates the identification of small amounts of proteins and their chemical modifications from complex biological samples. This resource allows Lunenfeld-Tanenbaum researchers and their collaborators to systematically characterize the variety of multi-protein assemblies present in normal cells and the changes in these protein complexes and their post-translational modifications that characterize the cellular response to different types of disease.

PI Contact: Dr. Anne-Claude Gingras
Facility contact: Brett Larsen


R

Robotics and High Throughput Screening Facility
robotics.lunenfeld.ca

This facility assists in the development of chemical and cellular assays for a variety of high-throughput experiments, and gives researchers access to a range of advanced robotic and analysis instrumentation as well as chemical and RNAi libraries. These technologies enable Lunenfeld-Tanenbaum as well as other Canadian scientists to probe the molecular pathways involved in myriad aspects of cellular, developmental and disease biology, and as a means to discover key compounds for drug discovery. 
 

PI contacts: Dr. Jeff Wrana and Dr. Jim Dennis 
Facility contact: Alessandro Datti 

 


 

 

 
   

 

 

 

  
  
    
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