High-Throughput Imaging Facility at Samuel Lunenfeld Research Institute

Leica Inverted and Dissecting

Inverted A basic imaging system with a camera for fluorescence (Hamamatsu) and second colour camera for histology. The cameras can be moved between two microscopes, an upright Leica dissecting microscope and an inverted Leica microscope equipped with various filter blocks. A Hg-lamp provides the light source and the system acquisition runs on Volocity software.
Location 865 (NW)
System Type Histo-fluorescence (camera-based)
Model a) Leica inverted b) Leica dissecting
Microscope a) Leica DMIRBE b) Leica MZFL111
Lenses a) Dry 2.5, 5, 10, 20, 40, 63 b) variable
Vibration isolation No
Stage Manual xy, Slide/dish adaptors
Z-focus Non-integrated
Illumination a) Hg lamp ebq100 b) Hg lamp HBO100
Filtering a) Multiple cubes b) GFP/Red
Detector Fluor camera: Hamamatsu C4742-95
Color camera:QImaging Micropublisher
Pinhole -
Software Volocity / Xp
Offline analysis Volocity
Other features