The natural SFV life cycle

Before explaining the vector system, here is a brief outline of the natural virus life cycle. For an excellent review of alpha viruses, see STRAUSS and STRAUSS

SFV has a plus strand (i.e protein coding) RNA genome of 11442nuc. (exluding the 5<92> cap and polyA tail). The 42S SFV RNA genome sequence can be downloaded here 

Now review how to make recombinant virus