|
For a simple, general picture of what replicon and helper vectors look like, go here
**All the vectors are Amp resistant**
The Table below describes all the plasmids and provides links to the publications describing them, their full sequence and schematic diagrams for each one. You can also download a PDF file containing all the Schematic diagrams + all the sequences together here . Also, here is a sequence file for the complete SFV genome if anyone needs it.
SFV Vectors
Type
|
Plasmid
|
Description
|
Ref
|
Sequence
|
Schematic diagram
|
| Helper |
pSCAHelper |
Expresses structural proteins for viral packaging |
JBC 1998. |
pSCAHelper |
pSCAHelper |
| Replicon |
pSCA1 |
Clone your gene of interest in this vector |
JBC 1998. |
pSCA1 |
pSCA1 |
| Replicon |
pSCAb |
LacZ cloned into pSCA1 |
JBC 1998. |
pSCAb |
pSCAb not available |
| Replicon |
pSMART2a |
Souped up version of pSCA1 with better cloning site, translational enhancer and N-terminal Flag and His tags |
IVOS 2004 |
pSMART2a |
pSMART2a |
| Replicon |
pSMART2b |
Same as 2a but cloning sites in different reading frame |
IVOS 2004 |
pSMART2b |
pSMART2b |
| Replicon |
pSMARTlacZ |
LacZ cloned into 2b |
IVOS 2004 |
|
|
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