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SFV Expression System
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SFV virus crystal structure
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The initial SFV expression system we developed was published in JBC 1998 Modifications that facilitate protein purification are described in a paper in IOVS 2004 in the vectors section.
Advantages
- Simple and fast - transfect two plasmids, harvest virus 1-2days later. No recombination required.
- Wide host range - allows for appropriate post-translational modifications.
- High protein output - can generate as much as 25% of total cellular protein
- Safe (level 2 procedures: gloves, lab coat, TC hood, bleach).
- virus components on two separate plasmids
- coat E2-E3 fusion protein contains three mutations, so virus only infects after activation by chymotrypsin treatment. Even if plasmids recombine, still need three reversions to obtain wild type virus. Never been detected.
How does it work?
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