High-Throughput Imaging Facility at Samuel Lunenfeld Research Institute

Nikon Confocal

Microscopes ยป Nikon Confocal
Nikon Confocal
Our newest confocal on an inverted microscope with integrated xy motorized stage. Capable of imaging multi-labelled specimens with overlapping emission. Simultaneous detection of 3 emission ranges/channels together with a transmitted light image (using either dry or oil high-NA condensor). Equipped with 408 UV, multi-line Argon (458/476/488/514), 568 and 635 nm lasers. An external spectral detector can provide live readout of emission over 32 channels. Acquisition software performs spectral unmixing, FRAP, FRET, and montage acquisition over multiple xyz fields and time. Powerful NIS-Elements analysis software.

Location 865 (SE)
System Type Confocal laser scanner (galvo-scanner/PMT)
Model Nikon C1si
Microscope Nikon Ti-E inverted
Lenses Dry 2, 4, 10, 20VC/0.75, Oil 60VC/1.4
Vibration isolation Yes
Stage Motorized, Slide/dish holder, Software-integrated
Z-focus Z-stage
Illumination a) C-HGFIE lamp
b) 488nm Kr/Ar laser plus other lines
c) 405, 568, 633nm lasers
Filtering a) Several imaging cubes
b) si spectral detector (400-750nm readout)
c) 3 viewing cubes
Detector a) Standard 3-ch PMT
b) Spectral 32-ch PMT
c) Transmitted light detector
Pinhole 3-position
Software EZ-C1 /Xp, Elements (analysis)
Offline analysis Elements license
Other features FRAP, FRET modules
multi-point montage
spectral un-mixing