OPTIMA

Quorum Spinning Disc

Microscopes » Quorum Spinning Disc
Nikon Confocal
The Quorum Modified Yokogawa Spinning Head, Hamamatsu EMCCD ultrasensitive camera, cartridges with fast filter wheels, and top stage environment chamber make this system optimized for live imaging, time lapse imaging. The spinning disc’s fixed pinholes are optimized for the 63x objective. Live Time FRAP, Photoactivation, Uncagging is possible using the Mosaic Digital Diaphragm System attached. Images can be further improved by Huygens or Volocity deconvolution available on the OFF Line computer station. Volocity’s Spectral separation can be utilized...

Location 6-1001-3 (Microscope Room B)
System Type Spinning Disc Confocal
Model Modified Yokogawa CSU10 scanner
Microscope Leica DMI 6000 inverted, motorized
Lenses Dry 5/0.15, 10/0.40, 20/0.70, 40/0.85 LWD, 40/0.60 Oil, 40/1.25, 63/1.4, 100/1.4
Vibration isolation Yes
Stage Motorized xyz, Slide/Plate/dish holder, Software-integrated, stage top environmental chamber
Z-focus microscope or piezo and autofocus
Illumination a) Halogen 12V 100W
b) EXFO X-Cite 120
c) Pumped Diode lasers: 405/460, 442/470, 491/525, 561/593 and 620, 642/700
Filtering a) 2 Filter Wheel Cartridges for lasers
b) eye piece viewing filter cubes
c) DIC
Detector Hamamatsu C9100-13 EM CCD Camera
Pinhole Fixed
Software Volocity/ Win XP 32
Offline analysis Volocity Visualization, Quantitation, and Restoration, Huygens deconvolution and Imaris modules/Win7 64
Other features FRAP, photoactivation, uncaging, stage top environmental chamber, multi point acquisition, multi frame scan, spectral unmixing