OPTIMA

Olympus Optigrid inverted

Microscopes » Olympus Optigrid inverted
Olympus inverted
This Volocity run system uses structured illumination to collect confocal like quality images. Faster, widefield (also for live cell imaging) acquisition followed by the Huygens or Volocity deconvolution is possible as well as spectral separation. Olympus L shaped illuminator provides very well corrected light path. The system has also been used for acquisition of large, multi-frame, one plane, scanned-stitched images. The system is capable of confocal stereology/image analysis using Visiopharm’s NewCAST/VisioMorph software.

Location 6-1001-2 (Microscope Room A)
System Type WF/Structured Illumination and Deconvolution
Model OptiGrid on IX81 and Huygens Deconvolution
Microscope Olympus IX81 inverted, motorized
Lenses Dry 10/0.4, 20/0.75, 40/0.95, Oil 40/1.3,60/1.45, 100/1.4
Vibration isolation Yes
Stage Motorized xyz, Slide/Plate holder, Software-integrated
Z-focus microscope and autofocus
Illumination a) Halogen 12V 100W
b) EXFO X-Cite Exacte
Filtering a) filter cubes: 377/447, 436/480, 482/536, 500/535, 562/624, 620/700
b) triple bandpas (blue/green/red) for eyepiece viewing
c) DIC
Detector Hamamatsu OrcaR2 CCD Camera
Pinhole -
Software Volocity/ Win XP 32
Offline analysis Volocity Visualization, Quantitation, and Restoration, Huygens deconvolution and Imaris modules/Win7 64/td>
Other features multi point acquisition, multi frame scan,
spectral unmixing, fluorescent stereology capable