High-Throughput Imaging Facility at Samuel Lunenfeld Research Institute

Olympus Upright

Microscopes ยป Olympus Upright
In Cell 6000 An upright Olympus microscope system with automated lens and filter selection that is also equipped with an Optigrid module to collect confocal-like images based on structured illumination microscopy (SIM). There is a camera for fluorescence (Hamamatsu) running on Volocity software, as well as a second colour camera for histology that can be used with Olympus DP Controller software.
Location 865 (SW)
System Type Optigrid-SIM/histo-fluor (camera-based)
Model Olympus
Microscope Olympus BX61
Lenses Dry 4,10,20/0.75 Oil 40x/1.3, 60x/1.42, 100x/1.4
Vibration isolation Yes
Stage Manual xy, Slide spring clip
Z-focus Integrated objective z-drive
Illumination EXFO X-Cite 120
Filtering DAPI, FITC, TxRed, CFP, YFP, BF-brightfield
Detector a) Fluor camera: Hamamatsu DP71 camera
b) Color camera: Olympus DP71
Pinhole -
Software Volocity / Xp
DP Controller
Offline analysis
Other features QIOPTIQ Optigrid
Structured illumination Microscopy (SIM)
Deconvolution